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Characterization of the novel role of NinaB orthologs from Bombyx mori and Tribolium castaneum

文献类型： 外文期刊

作者： Chai, Chunli 1 ; Xu, Xin 1 ; Sun, Weizhong 2 ; Zhang, Fang 1 ; Ye, Chuan 1 ; Ding, Guangshu 1 ; Li, Jiantao 1 ; Zhong, Gu 1 ;

作者机构： 1.Southwest Univ, Coll Biotechnol, Minist Agr & Rural Affairs, State Key Lab Silkworm Genome Biol,Key Lab Sericu, Chongqing 400715, Peoples R China

2.Southwest Univ, Coll Anim Sci & Technol, Chongqing 400715, Peoples R China

3.Zhejiang Univ, Life Sci Inst, Hangzhou 310058, Zhejiang, Peoples R China

4.Zhejiang Univ, Innovat Ctr Cell Signaling Network, Hangzhou 310058, Zhejiang, Peoples R China

5.Southwest Univ, Coll Plant Protect, Chongqing 400715, Peoples R China

6.Sichuan Acad Agr Sci, Sericulture Res Inst, Chengdu 610066, Sichuan, Peoples R China

7.Case Western Reserve Univ, Sch Med, Dept Pharmacol, Cleveland, OH 44106 USA

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.714； 五年影响因子：4.953 ）

ISSN： 0965-1748

年卷期： 2019 年 109 卷

页码：

收录情况： SCI

摘要： Carotenoids can be enzymatically converted to apocarotenoids by carotenoid cleavage dioxygenases. Insect genomes encode only one member of this ancestral enzyme family. We cloned and characterized the ninaB genes from the silk worm (Bombyx mori) and the flour beetle (Tribolium castaneum). We expressed BmNinaB and TcNinaB in E. coli and analyzed their biochemical properties. Both enzymes catalyzed a conversion of carotenoids into cis-retinoids. The enzymes catalyzed a combined trans to cis isomerization at the C11, C12 double bond and oxidative cleavage reaction at the C15, C15' bond of the carotenoid carbon backbone. Analyses of the spatial and temporal expression patterns revealed that ninaB genes were differentially expressed during the beetle and moth life cycles with high expression in reproductive organs. In Bombyx mori, ninaB was almost exclusively expressed in female reproductive organs of the pupa and adult. In Tribolium castaneum, low expression was found in reproductive organs of females but high expressions in male reproductive organs of the pupa and imagoes. We performed RNAi experiments to characterize the role of NinaB in insect reproduction. We observed that RNAi treatment significantly decreased the expression levels of BmninaB and TcninaB and reduced the egg laying capacity of both insects. Together, our study revealed that NinaB's unique enzymatic properties are well conserved among insects and implicate NinaB function in insect reproduction.

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