Comparative transcriptome analysis of cold-tolerant and -sensitive asparagus bean under chilling stress and recovery
文献类型: 外文期刊
作者: Miao, Mingjun 1 ; Tan, Huaqiang 3 ; Liang, Le 1 ; Huang, Haitao 4 ; Chang, Wei 2 ; Zhang, Jianwei 1 ; Li, Ju 2 ; Tang, Yi 1 ; Li, Zhi 2 ; Lai, Yunsong 1 ; Yang, Liang 2 ; Li, Huanxiu 1 ;
作者机构: 1.Sichuan Agr Univ, Coll Hort, Chengdu, Sichuan, Peoples R China
2.Sichuan Acad Agr Sci, Hort Res Inst, Chengdu, Sichuan, Peoples R China
3.Chengdu Acad Agr & Forestry Sci, Chengdu, Sichuan, Peoples R China
4.Mianyang Acad Agr Sci, Mianyang, Sichuan, Peoples R China
关键词: Asparagus bean; Sesquipedialis; RNA-seq; Cold stress; Recovery; Transcriptome
期刊名称:PEERJ ( 影响因子:3.061; 五年影响因子:3.537 )
ISSN: 2167-8359
年卷期: 2022 年 10 卷
页码:
收录情况: SCI
摘要: Background. Low temperature is a type of abiotic stress that threatens the growth and yield of asparagus bean. However, the key genes and regulatory pathways involved in low temperature response in this legume are still poorly understood. Methodology. The present study analyzed the transcriptome of seedlings from two asparagus bean cultivars-Dubai bean and Ningjiang 3-using Illumina RNA sequencing (RNA-seq). Correlations between samples were determined by calculating Pearson correlation coefficients (PCC) and principal component analysis (PCA). Differentially expressed genes (DEGs) between two samples were identified using the DESeq package. Transcription factors (TF) prediction, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of DEGs were also performed. Results. Phenotypes and physiological indices indicated that Ningjiang 3 seedlings tolerated cold better than Dubai bean seedlings, in contrast to adult stage. The transcriptome dynamics of the two cultivars were closely compared using Illumina RNA-seq following 0, 3,12, and 24 h of cold stress at 5 degrees C and recovery for 3 h at 25 degrees C room temperature. Global gene expression patterns displayed relatively high correlation between the two cultivars (>0.88), decreasing to 0.79 and 0.81, respectively, at 12 and 24 h of recovery, consistent with the results of principal component analysis. The major transcription factor families identified from differentially expressed genes between the two cultivars included bHLH, NAC, C2H2, MYB, WRKY, and AP2/ERF. The representative GO enrichment terms were protein phosphorylation, photosynthesis, oxidation-reduction process, and cellular glucan metabolic process. Moreover, KEGG analysis of DEGs within each cultivar revealed 36 transcription factors enriched in Dubai bean and Ningjiang 3 seedlings under cold stress. Conclusions. These results reveal new information that will improve our understanding of the molecular mechanisms underlying the cold stress response of asparagus bean and provide genetic resources for breeding cold-tolerant asparagus bean cultivars.
- 相关文献
作者其他论文 更多>>
-
Foliar application of salicylic acid inhibits the cadmium uptake and accumulation in lettuce (Lactuca sativa L.)
作者:Tang, Wen;Liang, Le;Lin, Lijin;Huang, Zhi;Sun, Bo;Sun, Guochao;Li, Huanxiu;Tang, Yi;Xie, Yongdong;Li, Xiaomei;Li, Xiaomei;Tu, Lihua
关键词:salicylic acid; lettuce; cadmium; ion transporter gene; antioxidant enzyme
-
Interspecific rootstocks improve the low-temperature resistance of bitter gourd through sucrose and nitrogen metabolism regulation
作者:Liang, Le;Dong, Yiping;Tang, Wanjia;Zhang, Ran;Peng, Xuemei;Lian, Huashan;Li, Huanxiu;Tang, Yi;Li, Xiaomei;Li, Xiaomei
关键词:Bitter gourd; Grafting; Low temperature; Sucrose metabolism; Nitrogen metabolism
-
Variation in the Main Health-Promoting Compounds and Antioxidant Capacity of Three Leafy Vegetables in Southwest China
作者:Zhang, Yi;Huang, Wenli;Zhang, Chenlu;Huang, Huanhuan;Yang, Shihan;Wang, Yiqing;Huang, Zhi;Tang, Yi;Li, Huanxiu;Zhang, Fen;Sun, Bo;Li, Xiaomei;Li, Xiaomei;Lian, Huashan
关键词:malabar spinach; amaranth; sweet potato; phenolic compounds; antioxidants; antioxidant capacity
-
A point mutation in MC06g1112 encoding FLOWERING LOCUS T decreases the first flower node in bitter gourd (Momordica charantia L.)
作者:Zhong, Jian;Dong, Jichi;Liu, Jia;Zhong, Chunfeng;Cheng, Jiaowen;Hu, Kailin;Zhong, Jian;Miao, Mingjun;Cui, Junjie;Hu, Fang
关键词:bitter gourd; first flower node; quantitative trait locus; fine-mapping; FLOWERING LOCUS T
-
Efficient plant genome engineering using a probiotic sourced CRISPR-Cas9 system
作者:Zhong, Zhaohui;Tang, Zhongjie;Xiang, Shuyue;Huang, Lan;He, Yao;Fan, Tingting;Liu, Shishi;Zheng, Xuelian;Huang, Jian;Zhang, Yong;Zhong, Zhaohui;Zheng, Xuelian;Zhang, Yong;Liu, Guanqing;Zhang, Tao;Liu, Guanqing;Zhang, Tao;Liu, Guanqing;Zhang, Tao;Yang, Liang;Yang, Liang;Qi, Yiping;Qi, Yiping
关键词:
-
Molecular cytogenetic maps provide new insights into the differences between hybrid Citrus microcarpa and lemons *
作者:Song, Shipeng;You, Xixi;Zheng, Dengyue;Tang, Yi;Chen, Chunli;Song, Shipeng;You, Xixi;Zheng, Dengyue;Tang, Yi;Chen, Chunli;He, Li
关键词:Fluorescence in situ hybridization (FISH); Oligo pool; Chromosomal identification; CMA bands; Calamondin; Lemon
-
Comparative transcriptome analysis revealed differential gene expression involved in wheat leaf senescence between stay-green and non-stay-green cultivars
作者:Li, Qing;Yang, Huai;Guo, Jingwei;Huang, Qianglan;Zhong, Shengfu;Tan, Feiquan;Ren, Tianheng;Li, Zhi;Chen, Chen;Luo, Peigao;Li, Qing;Guo, Jingwei
关键词:wheat (Triticum aestivum L; ); leaf senescence; photosynthesis; chlorophyll; antioxidant enzyme; transcriptome