Effects of bacterial haemorrhagic septicemia on the immune response of Leiocassis longirostris by RNA-Seq and microRNA-Seq
文献类型: 外文期刊
作者: Zhou, Jian 1 ; Zhao, Han 1 ; Zhang, Lu 1 ; Ye, Xianlin 2 ; Wang, Zhipeng 2 ; Li, Qiang 1 ; Ke, Hongyu 1 ; Zhao, Gang 1 ; Du, 1 ;
作者机构: 1.Sichuan Acad Agr Sci, Fisheries Inst, Chengdu 611731, Sichuan, Peoples R China
2.Sichuan Acad Agr Sci, Chengdu 610066, Sichuan, Peoples R China
3.Sichuan Agr Univ, Coll Anim Sci & Technol, Chengdu 611130, Sichuan, Peoples R China
关键词: Leiocassis longirostris; Aeromonas hydrophila; Immune response; mRNA and miRNA sequencing
期刊名称:COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS ( 影响因子:2.674; 五年影响因子:2.941 )
ISSN: 1744-117X
年卷期: 2020 年 34 卷
页码:
收录情况: SCI
摘要: Leiocassis longirostris is a common fish variety that is widely cultivated in China, during the breeding process however, it is highly susceptible to bacterial haemorrhagic septicemia, which can cause great economic loss for farmers. To understand the immune responses of L. longirostris to Aeromonas hydrophila infection, Illumina sequencing was employed to identify changes in the mRNA and miRNA in spleen tissue. In this study, a total of 92.16 and 95.61 million (M) high-quality transcriptome reads were generated from the control group (CG) and experimental group (EG) spleen samples, respectively, and 207 up-regulated and 185 down-regulated genes were identified. These genes were enriched in 29 GO terms and 30 KEGG pathways (P <= 0.05), including cytokine-cytokine receptor interaction and complement and coagulation cascades, with 17 up-regulated genes and 12 down-regulated genes related to immune responses in the EG relative to the CG. Based on the zebrafish genome, miRNA-seq identified a total of 343 miRNAs, of which 15 were up-regulated and 10 were down-regulated (fold-change >= 2 or <= 0.5 and P <= 0.05). Target gene prediction and KEGG enrichment analysis revealed that all of the target genes were concentrated in 13 pathways associated with immune response, including the mTOR signaling pathway and the TGF-beta signaling pathway. The expression patterns of 8 differentially expressed genes and 4 miRNAs involved in immune response were validated by quantitative real-time RT-PCR. These results have provided valuable insights into the molecular mechanisms underlying the immune response of L. longirostris to bacterial haemorrhagic septicemia.
- 相关文献
作者其他论文 更多>>
-
The background adaptation of the skin color in the loach Paramisgurnus dabryanus
作者:Luo, Wei;Zhang, Shoudong;Zhang, Xiaoyang;Zhang, Yibo;Chen, Pengyu;Jiang, Jun;Yang, Shiyong;Yang, Song;Du, Zongjun;Wang, Tianzhu;Guo, Zhonggang;Xiong, Yinlin;Xu, Zhou
关键词:Background adaptation; Skin color; Discoloration mechanism; Paramisgurnus dabryanus
-
Water extract of Fagopyrum dibotrys (D. Don) Hara straw increases selenium accumulation in peach seedlings under selenium-contaminated soil
作者:Li, Zhiyu;Xiao, Yunying;Zhou, Kexuan;Jin, Xin;Li, Wan;Li, Wanzhi;Zhang, Lu;Wang, Jin;Lin, Lijin;Hu, Rongping
关键词:Fagopyrum dibotrys (D. Don) Hara; peach; selenium; straw water extract
-
Effect of tartary buckwheat and highland barley flours on the quality of glutinous rice cakes determined by nutritional composition, physical structure, sensory perception and flavour
作者:Zhou, Mei;Li, Yanmi;Yuan, Ziqi;Ren, Yuanhang;Zeng, Qingchen;Qi, Anyin;Wu, Xiaoyong;Wu, Qi;Ye, Xueling;Liu, Changying;Fan, Yu;Hu, Yichen;Peng, Lianxin;Xiang, Dabing;Zou, Liang;Zhao, Gang;Wan, Yan;Luo, Liming;Jin, Tao;Wan, Yan;Huang, Jingwei;Cao, Xiaoning;Shi, Zhiqiang
关键词:Coarse-cereal glutinous rice cake; quality; texture characteristics; volatile flavour
-
Comparative transcriptome and genome analysis unravels the response of Tatary buckwheat root to nitrogen deficiency
作者:Liu, Changying;Qiu, Qingcheng;Zou, Bangxing;Wu, Qi;Ye, Xueling;Wan, Yan;Huang, Jingwei;Wu, Xiaoyong;Sun, Yanxia;Yan, Huiling;Fan, Yu;Jiang, Liangzhen;Zheng, Xiaoqin;Zhao, Gang;Zou, Liang;Xiang, Dabing;Zou, Bangxing
关键词:Nitrogen; Root; Transcriptome; Tartary buckwheat; Whole-genome re -sequencing
-
Integrated analysis of how gender and body weight affect the intestinal microbial diversity of Gymnocypris chilianensis
作者:Zhao, Zhongmeng;Zhao, Han;Zhang, Lu;Huang, Zhipeng;Ke, Hongyu;Liu, Ya;Duan, Yuanliang;Li, Huadong;Li, Qiang;Wang, Xiongyan
关键词:
-
Single-nuclei RNA-seq reveals skin cell responses to Aeromonas hydrophila infection in Chinese longsnout catfish Leiocassis longirostris
作者:Mou, Cheng-Yan;Zhang, Lu;Zhao, Han;Huang, Zhi-Peng;Duan, Yuan-Liang;Zhao, Zhong-Meng;Ke, Hong-Yu;Du, Jun;Li, Qiang;Zhou, Jian
关键词:single nuclei RNA-sequencing; fish skin cell; immune response; Aeromonas hydrophila; cell expansion
-
Effect of total dissolved gas supersaturation and flow velocity on survival and swimming ability of juvenile Schizothorax prenanti
作者:Yuan, Quan;Yuan, Quan;Li, Kefeng;Wang, Yuanming;Liang, Ruifeng;Du, Jun
关键词:Flow velocity; Schizothorax prenanti; survival time; swimming ability; TDG supersaturation