文献类型： 外文期刊

作者： Yang, S. ¹ ; Wu, H. ¹ ; He, K. ¹ ; Yan, T. ² ; Zhou, J. ² ; Zhao, L. L. ¹ ; Sun, J. L. ¹ ; Lian, W. Q. ¹ ; Zhang, D. M. ¹ ; Du, ¹ ;

作者机构： 1.Sichuan Agr Univ SICAU, Coll Anim Sci & Technol, Chengdu 611130, Sichuan, Peoples R China

2.Sichuan Acad Agr Sci, Fisheries Inst, Chengdu 611731, Sichuan, Peoples R China

3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China

关键词： Teleost; Hypoxic stress; AMP activated protein kinase; Monocarboxylate transporter; Lactate dehydrogenase

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：7.963； 五年影响因子：7.842 ）

ISSN： 0048-9697

年卷期： 2019 年 666 卷

页码：

收录情况： SCI

摘要： To study adaptation of largemouth bass (Micropterus salmoides) to hypoxic stress, we investigated physiological responses and lactate metabolism of the fish under acute hypoxia. The objectives of this study were to (a) observe changes in glucose, glycogen, and lactate content; (b) detect the activity of lactate dehydrogenase (LDH) in serurn, brain, heart, and liver tissues; and (c) quantify the dynamic gene expression of AMP activated protein kinase alpha (AMPK alpha), hypoxia-inducible factor-1 alpha (HIF-1 alpha) monocarboxylate transporter 1 (MCT1), monocarboxylate transporter 4 (MCT4), and lactate dehydrogenase-a (LDHa) following exposure to hypoxia. The fish were subjected to two hypoxia stresses (dissolved oxygen [DO] 120 +/- 0.2 mg/L and 3.50 +/- 0.3 mg/L, respectively) for 24 h. Our results showed that hypoxic stress significantly increased the decomposition of liver glycogen and significantly increased the concentration of blood glucose; however, the muscle glycogen content was not significantly decreased, which indicates that liver glycogen was the main energy source under acute hypoxia. Moreover, hypoxia led to accumulation of a large amount of lactic acid in tissues, possibly due to the activity of lactic acid dehydrogenase, but this process was delayed in the heart and brain relative to the liver. Additionally, hypoxia induced the expression of AMPK alpha, HIF-1 alpha, MCT1, MCT4, and LDHa, suggesting that glycometabolism had switched from aerobic to anaerobic. Our results contribute to a better understanding of the molecular mechanisms of the response to hypoxia in largemouth bass. (C) 2019 Published by Elsevier B.V.