Genome-Wide Distribution of Novel Ta-3A1 Mini-Satellite Repeats and Its Use for Chromosome Identification in Wheat and Related Species
文献类型: 外文期刊
作者: Lang, Tao 1 ; Li, Guangrong 1 ; Yu, Zhihui 1 ; Ma, Jiwei 1 ; Chen, Qiheng 1 ; Yang, Ennian 2 ; Yang, Zujun 1 ;
作者机构: 1.Univ Elect Sci & Technol China, Ctr Informat Biol, Sch Life Sci & Technol, Chengdu 611731, Sichuan, Peoples R China
2.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Sichuan, Peoples R China
关键词: fluorescence in situ hybridization; mini-satellite; tandem repeats; wheat
期刊名称:AGRONOMY-BASEL ( 影响因子:3.417; 五年影响因子:3.64 )
ISSN: 2073-4395
年卷期: 2019 年 9 卷 2 期
页码:
收录情况: SCI
摘要: A large proportion of the genomes of grasses is comprised of tandem repeats (TRs), which include satellite DNA. A mini-satellite DNA sequence with a length of 44 bp, named Ta-3A1, was found to be highly accumulated in wheat genome, as revealed by a comprehensive sequence analysis. The physical distribution of Ta-3A1 in chromosomes 3A, 5A, 5B, 5D, and 7A of wheat was confirmed by nondenaturing fluorescence in situ hybridization (ND-FISH) after labeling the oligonucleotide probe. The analysis of monomer variants indicated that rapid sequence amplification of Ta-3A1 occurred first on chromosomes of linkage group 5, then groups 3 and 7. Comparative ND-FISH analysis suggested that rapid changes occurred in copy number and chromosomal locations of Ta-3A1 among the different species in the tribe Triticeae, which may have been associated with chromosomal rearrangements during speciation and polyploidization. The labeling and subsequent use of Ta-3A1 by ND-FISH may assist in the precise identification and documentation of novel wheat germplasm engineered by chromosome manipulation.
- 相关文献
作者其他论文 更多>>
-
Whole genome doubling-induced the enrichment of H3K27me3 in genes carrying specific TEs in Aegilops tauschii
作者:Guo, Hongwei;Zhang, Guoyan;Zhou, Min;Wan, Min;Zhu, Bo;Zeng, Deying;Zeng, Zixian;Zhang, Guoyan;Zhu, Bo;Zeng, Deying;Zeng, Zixian;Yang, Zujun
关键词:whole-genome doubling; Aegilops tauschii; transcriptome; transposable elements
-
Development of a Set of Wheat-Rye Derivative Lines from Hexaploid Triticale with Complex Chromosomal Rearrangements to Improve Disease Resistance, Agronomic and Quality Traits of Wheat
作者:Wang, Tingting;Li, Guangrong;Jiang, Chengzhi;Zhou, Yuwei;Yang, Zujun;Yang, Ennian;Li, Jianbo;Zhang, Peng;Dundas, Ian
关键词:FISH; rye; wheat; chromosome rearrangement; disease resistance
-
Molecular and Cytogenetic Identification of Wheat-Thinopyrum intermedium Double Substitution Line-Derived Progenies for Stripe Rust Resistance
作者:Li, Guangrong;Chen, Qiheng;Jiang, Wenxi;Zhang, Ahui;Yang, Zujun;Yang, Ennian
关键词:chromosome translocation; ND-FISH; Oligo-FISH painting; rust resistance; Thinopyrum intermedium; wheat
-
A major yellow rust resistance QTL on chromosome 6A shows increased frequency in recent Norwegian spring wheat cultivars and breeding lines
作者:Lin, Min;Lillemo, Morten;Dieseth, Jon Arne;Alsheikh, Muath;Yang, Ennian;Holzapfel, Josef;Schuermann, Friederike;Morales, Laura;Michel, Sebastian;Buerstmayr, Hermann;Bhavani, Sridhar
关键词:
-
Molecular and cytogenetic dissection of stripe rust resistance gene Yr83 from rye 6R and generation of resistant germplasm in wheat breeding
作者:Li, Guangrong;Zhang, Yao;Yang, Zujun;Li, Jianbo;Zhang, Peng;Ma, Qiang;Yang, Ennian;Dundas, Ian
关键词:FISH; rye; wheat; stripe rust; Yr83
-
Location of Tandem Repeats on Wheat Chromosome 5B and the Breakpoint on the 5BS Arm in Wheat Translocation T7BS.7BL-5BS Using Single-Copy FISH Analysis
作者:Zhang, Wei;Tang, Zongxiang;Luo, Jie;Fu, Shulan;Li, Guangrong;Yang, Zujun;Yang, Manyu;Yang, Ennian
关键词:single-copy FISH; ND-FISH; tandem repeats; wheat translocation; breakpoint
-
Genetic Diversity Assessment of the International Maize and Wheat Improvement Center and Chinese Wheat Core Germplasms by Non-Denaturing Fluorescence In Situ Hybridization
作者:Yang, Manyu;Yang, Wuyun;Yang, Ennian;Yang, Manyu;Yang, Wuyun;Yang, Ennian;Yang, Manyu;Yang, Wuyun;Yang, Ennian;Yang, Zujun
关键词:wheat; ND-FISH; genetic diversity; chromosomal translocation