文献类型： 外文期刊

作者： Shi, Jun ¹ ; Huang, Tingyou ¹ ; Xiang, Zufen ¹ ; Peng, Tao ¹ ; Shi, Shoupei ¹ ; Liu, Dingyou ¹ ; Chu, Xudong ¹ ;

作者机构： 1.Mianyang Acad Agr Sci, Mianyang 621023, Sichuan, Peoples R China

关键词： Gene identification; Resistance analysis; Rice blast; Rice restorer line

期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）

ISSN： 1560-8530

年卷期： 2019 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Blast disease caused by the filamentous fungus Magnaporthe oryzae is one of the most serious rice diseases throughout the world. Mianhui357 (MH357) is an elite hybrid rice restorer line with high resistance to rice blast. To identify the major functional blast resistance genes in MH357, a disease assay was performed with 90 M. oryzae strains on MH357, Lijiangxin Tuan Heigu (LTH) and LTH-derived monogenic lines to determine their resistance levels. The disease phenotypes of MH357 were level 5 and level 3 symptoms for seedling blast and neck blast, respectively. MH357 had a blast resistance frequency of 83.3% to artificial inoculation and was classified as moderately resistant (MR) in the field, suggesting broad-spectrum resistance. The LTH monogenic lines with Pi2, Pi9, Pi5 and Pikm exhibited high blast resistance in Sichuan Province. Additionally, we performed expression profiling by reverse transcription PCR (RT-PCR) on previously reported blast resistance genes in MH357. Genes at the Pita, Pid2, Pia, Pi5 and Pik loci showed high expression. Furthermore, molecular markers and blast resistance gene cloning were used to identify the R genes in MH357. Our data suggested that the broad-spectrum blast R genes Pi5 and Pid2-357 contributed to the rice blast resistance of MH357, and that Pid2-357 was a novel functional allele conferring blast resistance. Thus, MH357 is a restorer line with broad-spectrum resistance containing multiple blast resistance genes, and is valuable for rice blast resistance breeding. (C) 2019 Friends Science Publishers