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Long-read sequencing reveals genomic structural variations that underlie creation of quality protein maize

文献类型： 外文期刊

作者： Li, Changsheng 1 ; Xiang, Xiaoli 2 ; Huang, Yongcai 2 ; Zhou, Yong 2 ; An, Dong 1 ; Dong, Jiaqiang 5 ; Zhao, Chenxi 6 ; L 1 ;

作者机构： 1.Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China

2.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Natl Key Lab Plant Mol Genet, Inst Plant Physiol & Ecol,Shanghai Inst Biol Sci, Shanghai 200032, Peoples R China

3.Sichuan Acad Agr Sci, Inst Biotechnol & Nucl Technol, Chengdu 610061, Peoples R China

4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

5.Rutgers State Univ, Waksman Inst Microbiol, 190 Frelinghuysen Rd, Piscataway, NJ 08854 USA

6.Univ Chinese Acad Sci, BGI Educ Ctr, Shenzhen 518083, Peoples R China

7.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China

8.Qingdao Agr Univ, Coll Agron, Qingdao 266109, Peoples R China

9.Montclair State Univ, Dept Biol, Montclair, NJ 07043 USA

10.Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA

期刊名称：NATURE COMMUNICATIONS （ 影响因子：14.919； 五年影响因子：15.805 ）

ISSN： 2041-1723

年卷期： 2020 年 11 卷 1 期

页码：

收录情况： SCI

摘要： Mutation of o2 doubles maize endosperm lysine content, but it causes an inferior kernel phenotype. Developing quality protein maize (QPM) by introgressing o2 modifiers (Mo2s) into the o2 mutant benefits millions of people in developing countries where maize is a primary protein source. Here, we report genome sequence and annotation of a South African QPM line K0326Y, which is assembled from single-molecule, real-time shotgun sequencing reads collinear with an optical map. We achieve a N50 contig length of 7.7 million bases (Mb) directly from long-read assembly, compared to those of 1.04Mb for B73 and 1.48Mb for Mo17. To characterize Mo2s, we map QTLs to chromosomes 1, 6, 7, and 9 using an F-2 population derived from crossing K0326Y and W64Ao2. RNA-seq analysis of QPM and o2 endosperms reveals a group of differentially expressed genes that coincide with Mo2 QTLs, suggesting a potential role in vitreous endosperm formation.

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